Droplet Digital PCR-Based Detection of Clarithromycin Resistance in Helicobacter pylori Isolates Reveals Frequent Heteroresistance
نویسندگان
چکیده
منابع مشابه
Fast and accurate quantitative detection of Helicobacter pylori and identification of clarithromycin resistance mutations in H. pylori isolates from gastric biopsy specimens by real-time PCR.
Rapid identification of patients infected with clarithromycin-resistant Helicobacter pylori without the need for culture can help to avoid useless prescriptions of clarithromycin. We developed and tested a routine real-time quantitative PCR assay dedicated to that purpose. One hundred ninety-six consecutive gastric biopsy specimens were examined by culture, histology performed by a trained phys...
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Background: Clarithromycin resistance in Helicbacter pylori has been found to be associated with point mutations in 23s rRNA gene leads to reduced affinity of the antibiotic to its ribosomal target or changing the site of methylation. The aim of this study was to determine the most important point mutations in 23s rRNA gene in H. pylori that are closely related to clarith-romycin resistance amo...
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The aim of this study was to determine the frequency of resistance to amoxycillin, tetracycline, metronidazole and clarithromycin in 282 Helicobacter pylori clinical isolates from Spain and to evaluate the evolution of resistance over the five years of this study. The overall percentage of resistance was 19.9% for metronidazole and 3.5% for clarithromycin. Resistance to metronidazole rose from ...
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BACKGROUND Clarithromycin therapy is effective in eradicating Helicobacter pylori. However, the resistance of H. pylori to clarithromycin is increasingly reported. The present study aimed to characterize the types of mutations present in the 23S rRNA genes of isolates of clarithromycin-resistant H. pylori from the UAE. METHODS Clarithromycin susceptibility of H. pylori isolates (n = 26) was d...
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Helicobacter pylori is a gastric pathogen that causes several gastroduodenal disorders such as peptic ulcer disease and gastric cancer. Eradication efforts of H. pylori are often hampered by antimicrobial resistance in many countries, including Vietnam. Here, the study aimed to investigate the occurrence of antimicrobial resistance among H. pylori clinical isolates across 13 hospitals in Viet...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2018
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.00019-18